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primary mouse anti human antibody against β tubulin  (Proteintech)


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    Proteintech primary mouse anti human antibody against β tubulin
    Figure 7. Trehalose inhibits 6-OHDA-induced activation of JNK, p38, and AMPK. (a–f) SH-SY5Y cells were incubated with or without trehalose (100 mM) and then treated or not with 6-OHDA (60 µM) for another 2 or 6 h. The levels of phosphorylated and total forms of JNK (a), p38 MAPK (b), ERK (c), AMPK (d), AKT (e), and 4EBP1 (f), together with loading controls (actin or <t>tubulin),</t> were assessed by immunoblotting, and the representative immunoblots are shown. Densitometry data are mean ± SD values from three independent experiments (* p < 0.05 vs. no treatment; # p < 0.05 vs. 6-OHDA).
    Primary Mouse Anti Human Antibody Against β Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+anti+human+antibody+against+%CE%B2+tubulin/beta+Tubulin+Antibody/pm39408988-392-39-45
    Average 96 stars, based on 2475 article reviews
    primary mouse anti human antibody against β tubulin - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways."

    Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms251910659

    Figure 7. Trehalose inhibits 6-OHDA-induced activation of JNK, p38, and AMPK. (a–f) SH-SY5Y cells were incubated with or without trehalose (100 mM) and then treated or not with 6-OHDA (60 µM) for another 2 or 6 h. The levels of phosphorylated and total forms of JNK (a), p38 MAPK (b), ERK (c), AMPK (d), AKT (e), and 4EBP1 (f), together with loading controls (actin or tubulin), were assessed by immunoblotting, and the representative immunoblots are shown. Densitometry data are mean ± SD values from three independent experiments (* p < 0.05 vs. no treatment; # p < 0.05 vs. 6-OHDA).
    Figure Legend Snippet: Figure 7. Trehalose inhibits 6-OHDA-induced activation of JNK, p38, and AMPK. (a–f) SH-SY5Y cells were incubated with or without trehalose (100 mM) and then treated or not with 6-OHDA (60 µM) for another 2 or 6 h. The levels of phosphorylated and total forms of JNK (a), p38 MAPK (b), ERK (c), AMPK (d), AKT (e), and 4EBP1 (f), together with loading controls (actin or tubulin), were assessed by immunoblotting, and the representative immunoblots are shown. Densitometry data are mean ± SD values from three independent experiments (* p < 0.05 vs. no treatment; # p < 0.05 vs. 6-OHDA).

    Techniques Used: Activation Assay, Incubation, Western Blot

    Related Articles

    Blocking Assay:

    Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
    Article Snippet: Equal amounts of denatured proteins from each sample were separated by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with 5% nonfat dried milk or bovine serum albumin (for anti-phosphoprotein antibodies), the membranes were incubated overnight at 4 °C with primary rabbit anti-human antibodies against PARP1, cleaved caspase-3, LC3B, JNK1/2, phospho-JNK1/2 (Thr183/Tyr185), ERK1/2, phospho-ERK1/2 (Thr202/Tyr204), p38 MAPK, phospho-p38 MAPK (Thr180/Tyr182), AMPKα1/2, phospho-AMPKα1/2 (Thr172), AKT, phospho-AKT (Ser473), 4EBP1, phospho-4EBP1 (Thr37/46), catalase, (all from Cell Signaling Technology, Beverly, MA, USA), SOD1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MAO-A (Abcam, Cambridge, UK), sequestosome 1/p62, β-actin (both from Novus Biologicals, Littleton, CO, USA), glyceraldehyde 3 phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, Waltham, MA, USA), or primary mouse anti-human antibody against β-tubulin (Proteintech, Rosemont, IL, USA). .. A peroxidase-conjugated goat anti-rabbit IgG (Jackson IP Laboratories, West Grove, PA, USA) and goat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) were used as secondary antibodies.

    Incubation:

    Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
    Article Snippet: Equal amounts of denatured proteins from each sample were separated by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with 5% nonfat dried milk or bovine serum albumin (for anti-phosphoprotein antibodies), the membranes were incubated overnight at 4 °C with primary rabbit anti-human antibodies against PARP1, cleaved caspase-3, LC3B, JNK1/2, phospho-JNK1/2 (Thr183/Tyr185), ERK1/2, phospho-ERK1/2 (Thr202/Tyr204), p38 MAPK, phospho-p38 MAPK (Thr180/Tyr182), AMPKα1/2, phospho-AMPKα1/2 (Thr172), AKT, phospho-AKT (Ser473), 4EBP1, phospho-4EBP1 (Thr37/46), catalase, (all from Cell Signaling Technology, Beverly, MA, USA), SOD1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MAO-A (Abcam, Cambridge, UK), sequestosome 1/p62, β-actin (both from Novus Biologicals, Littleton, CO, USA), glyceraldehyde 3 phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, Waltham, MA, USA), or primary mouse anti-human antibody against β-tubulin (Proteintech, Rosemont, IL, USA). .. A peroxidase-conjugated goat anti-rabbit IgG (Jackson IP Laboratories, West Grove, PA, USA) and goat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) were used as secondary antibodies.



    Similar Products

    primary mouse anti human antibody against β tubulin  (Proteintech)
    96
    Proteintech primary mouse anti human antibody against β tubulin
    Figure 7. Trehalose inhibits 6-OHDA-induced activation of JNK, p38, and AMPK. (a–f) SH-SY5Y cells were incubated with or without trehalose (100 mM) and then treated or not with 6-OHDA (60 µM) for another 2 or 6 h. The levels of phosphorylated and total forms of JNK (a), p38 MAPK (b), ERK (c), AMPK (d), AKT (e), and 4EBP1 (f), together with loading controls (actin or <t>tubulin),</t> were assessed by immunoblotting, and the representative immunoblots are shown. Densitometry data are mean ± SD values from three independent experiments (* p < 0.05 vs. no treatment; # p < 0.05 vs. 6-OHDA).
    Primary Mouse Anti Human Antibody Against β Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+anti+human+antibody+against+%CE%B2+tubulin/beta+Tubulin+Antibody/pm39408988-392-39-45
    Average 96 stars, based on 1 article reviews
    primary mouse anti human antibody against β tubulin - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 7. Trehalose inhibits 6-OHDA-induced activation of JNK, p38, and AMPK. (a–f) SH-SY5Y cells were incubated with or without trehalose (100 mM) and then treated or not with 6-OHDA (60 µM) for another 2 or 6 h. The levels of phosphorylated and total forms of JNK (a), p38 MAPK (b), ERK (c), AMPK (d), AKT (e), and 4EBP1 (f), together with loading controls (actin or tubulin), were assessed by immunoblotting, and the representative immunoblots are shown. Densitometry data are mean ± SD values from three independent experiments (* p < 0.05 vs. no treatment; # p < 0.05 vs. 6-OHDA).

    Journal: International journal of molecular sciences

    Article Title: Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways.

    doi: 10.3390/ijms251910659

    Figure Lengend Snippet: Figure 7. Trehalose inhibits 6-OHDA-induced activation of JNK, p38, and AMPK. (a–f) SH-SY5Y cells were incubated with or without trehalose (100 mM) and then treated or not with 6-OHDA (60 µM) for another 2 or 6 h. The levels of phosphorylated and total forms of JNK (a), p38 MAPK (b), ERK (c), AMPK (d), AKT (e), and 4EBP1 (f), together with loading controls (actin or tubulin), were assessed by immunoblotting, and the representative immunoblots are shown. Densitometry data are mean ± SD values from three independent experiments (* p < 0.05 vs. no treatment; # p < 0.05 vs. 6-OHDA).

    Article Snippet: 2024, 25, 10659 19 of 23 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MAO-A (Abcam, Cambridge, UK), sequestosome 1/p62, β-actin (both from Novus Biologicals, Littleton, CO, USA), glyceraldehyde 3 phosphate dehydrogenase (GAPDH; Thermo Fisher Scientific, Waltham, MA, USA), or primary mouse anti-human antibody against β-tubulin (Proteintech, Rosemont, IL, USA).

    Techniques: Activation Assay, Incubation, Western Blot